Fly Lines

GS strain number and vector version

Treating GS lines in molecular level, vector version must be determined. Version of GS vector carried by certain GS strain is correspond to it's strain number. Some part of GS line has altered genetic background and that also correspond to strain number.

Here is correspondence table of GS strain number and vector version they have.

Strain Number	Vector version	note
1 - 4999	GSV1	some lines have y[+] background
5000 - 5999	GSV2
6000 - 6999	GSV3
7000 - 7999	GSV2
8000 - 8999	GSV3
9000 - 19999	GSV6	rarely include X insertion
20000 - 29999	GSV7
50000 - 59999	GSV2

List of all mapped GS strains available at Resources page.

Lines used to establish GS Lines

All GS line are established by standard jump starter method. Strains used are;

category	Genotype/Strain name	GS number related	note
recipient	y[1] w[67c23]	all	all GS lines have genetic background of this line essentially
balancer	y[1] w[67c23]; wg[Sp-1]/SM1; Pr Dr/TM3, Sb Ser	autosomal insertion except 1 - 99 and 5000 - 6999	balancer used for autosomal insertion
balancer	Binsinscy	X insertion except 50000 - 59999	balancer used for X insertion
balancer	w[67c23]; wg[Sp-1]/SM1; Pr Dr/TM3, Sb Ser	autosomal insertion 1 - 99 and 5000 - 6999	balancer used for autosomal insertion
balancer	y[1] w[67c23]; Pr Dr/TM6, Sb Tb	varied	balancer used for insertion on distal tip of 3L
balancer	C(1)DX, y[1] f[1]	X insertion 50000 - 59999	balancer used for X insertion
balancer	y w; ci[D]/l(4)l[B]	4th chromosomal insertion	balancer used for 4th chromosome
Δ2-3	y[1] w[67c23]; ry[506] Sb[1] P{Δ2-3}99B/TM6, Ubx	all	originated from Bloomington #3612. replaced X and 2nd chromosome with recipient line's
jump starter	GS046	1 - 4999	bearing GSV1 on CyO. DGRC #20023
jump starter	II-02js	5000 - 5999 7000 - 7999 50000 - 59999	bearing GSV2 on CyO
jump starter	III-03js	6000 - 6999 8000 - 8999	bearing GSV3 on CyO
jump starter	CyO-VI-03js	9000 - 9133	bearing GSV6 on CyO
jump starter	X-VI-02js	9134 - 19999	bearing GSV6 on X
jump starter	CyO-VII-03js	20000 - 29999	bearing GSV6 on X

Lines related to GS system

Cre lines used to reverse GSV7 are;

Genotype	note
y[1] w[67c23] P{y[+mDint2]=Crey}1b; noc[Sco]/CyO	marked with y[+], X-linked. Bloomington #766
y[1] w[67c23]; P{y[+mDint2]=Crey}1b; D[*]/TM3, Sb[1]	marked with y[+], autosomal. Bloomington #851
y[1] w[67c23]; noc[Sco]/CyO, P{w[+mC]=Crew}DH1	marked with w[+], autosomal. Bloomington #1092

Bloomington #1092 is especially suitable for this purpose. See methods page for detail.

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